“Biochemist who loves traveling, exploring new places and trying different food. Loves dogs, nature and being outdoors!”
Senior Postdoctoral Fellow
Filamentous phages have been shown to have a positive impact on its pathogenic host by increasing antimicrobial resistance, motility or biofilm formation. More recently, it has also been shown that bacterial pathogens which have been infected by filamentous phages cause more severe disease progression in humans compared to non-infected bacteria.
One of my research goals is to understand the impact of filamentous prophages in bacterial host genomes. Making use of bioinformatics and microbiological tools, my group studies prophages in ESKAPE pathogens. We have recently discovered two filamentous phages in these species which contain genes with putative toxin functions, which we plan to characterise while also investigating the biology of the phages and their impact on the host (e.g. biofilm, capsule formation). Studying putative virulence factors and infection mechanisms is of crucial medical importance.
In addition to the impact of filamentous phages on the host, I am also fascinated by the peculiar biology of these phages: Instead of being released from the cytoplasm through lysis of the host cell, infected bacteria continue to grow while filamentous phages are assembled at the inner membrane of the bacterium and extruded across the cell envelope. We investigate the phage assembly process which includes the functional characterisation and structural elucidation.
2010 – 2014
Max Planck Institute for Developmental Biology
University of Tuebingen, Germany
PhD in Biochemistry (magna cum laude)
“The CCR4-NOT deadenylation complex functions in the Nonsense Mediated mRNA Decay pathway”
Dr. Elisa Izaurralde
2002 – 2004
University of Queensland, Australia
Bachelor of Biotechnology
(Second Upper Class Honours)
2018 – present
Zhejiang University-University of Edinburgh Institute
Postdoctoral Research Fellow
Research: Study of membrane proteins & bacteriophages
2015 – 2017
University of Hohenheim, Germany
Research: Structure-function relationship of membrane proteins with lipids.
2009 – 2010
University of Oxford, UK
Research: Correlation of HLA-types with HIV progression.
2008 – 2009
University College London, UK
Research: Understanding the effects of small chemical compounds on HIV life cycle.
Research: Constructing phage display library.
2004 – 2007
National University of Singapore, SG
Research: Understanding the role of SARM in C. rotundicauda.
Awards & Funding
China Postdoctoral Science Foundation, CN
“Elucidating the multi-functional role of Membrane-embedded molecular motors”
Grant number: 530000-X91902
University of Hohenheim, DE
“Investigating Protein-Lipid Interactions: The Crucial Role of the Lipid Bilayer Composition on Structure and Activity of Membrane Proteins”
University of Australia, AU
Summer Research Scholarship
Manohar P, Loh B, Namasivayam E, Loganathan A, Nachimuthu R, Leptihn S. (2022) A multiwell-plate Caenorhabditis elegans assay for assessing the therapeutic potential of Bacteriophages against Clinical Pathogens. Microbiology Spectrum Accepted.
Manohar P, Royam MM, Loh B, Bozdogan B, Nachimuthu R, Leptihn S. (2022) Synergistic Effects of Phage-Antibiotic Combinations against Citrobacter amalonaticus. ACS Infectious Diseases (Ahead of press)
Manohar P, Loh B, Namasivayam E, Loganathan A, Nachimuthu R, Leptihn S. (2022) A multiwell-plate Caenorhabditis elegans assay for assessing the therapeutic potential of Bacteriophages against Clinical Pathogens. bioRxiv.
Loh B, Zhang L, Hua X, Yu Y, Long M, Wang X, Manohar P, Nachimuthu R, Martins WMBS, Toleman MA, Leptihn S. (2021) Complete Genome of the Virulent Klebsiella pneumoniae Phage Geezett Infecting Multidrug-Resistant Clinical Strains. Microbiology Resource Announcements. 10(48) e00685-21.
Loh B, Wang X, Hua X, Luo J, Wen T, Zhang L, Ma L, Manohar P, Nachimuthu R, Grainge I, Yu Y, Leptihn S. (2021) Complete genomes of Bacteriophages Kaya, Guyu, Kopi and TehO that target clinical strains of Pseudomonas aeruginosa. Microbiology Resource Announcements. 10(48) e01043-21.
Wang X, Loh B, Altamirano FG, Yu Y, Hua X, Leptihn S. (2021) Colistin-phage combinations decrease antibiotic resistance in A. baumannii via changes in envelope architecture. Emerging Microbes & Infections. Nov 4:1-58.
Loh B, Wang X, Hua X, Chook HW, Ma L, Zhang L, Manohar P, Jin Y, Leptihn S. (2021) Complete Genome of the Lytic Bacteriophage Phab24 that infects Clinical Strains of the Nosocomial Pathogen Acinetobacter baumannii. Microbiology Resource Announcements. 10(40) e00669-21.
Wu Z, Zhang Y, Xu X, Ahmed T, Yang Y, Loh B, Leptihn S, Yan C, Chen J, Li B. (2021) The holin-endolysin lysis system of the OP2-like phage infecting Xanthomonas oryzae pv. oryzae. Viruses. 13(10), 1949
Das R, Kotra K, Singh P, Loh B, Leptihn S, Bajpai U. Alternative Treatment Strategies for Secondary Bacterial and Fungal Infections Associated with COVID-19. Infectious Diseases & Therapy (2021) 1–25.
Wang X, Loh B, Yu Y, Hua X, Leptihn S. (2021) Phage Resistance Mechanisms Increase Colistin Sensitivity in Acinetobacter baumannii. bioRxiv
Cui Z, Zhang Y, Kakar KU, Kong X, Li R, Loh B, Leptihn S, Li B (2021) Involvement of non-coding RNAs during infection of rice by Acidovorax oryzae. Environmental Microbiology Reports. 13(4), 540-554
Cai H, Zhu Y, Hu D, Li Y, Leptihn S, Loh B, Hua X, Yu Y (2021) Co-harboring of novel hybrid blaKPC−2 plasmid and ICE carrying Tn6203 in multidrug-resistant Pseudomonas aeruginosa. Frontiers in Microbiology. 12:674974.
Wang X, Zhang M, Loh B, Leptihn S, Ahmed T, Li B (2021) A Novel NRPS Cluster, Acquired by Horizontal Gene Transfer from Algae, Regulates Siderophore Iron Metabolism in Burkholderia seminalis R456. International Journal of Biological Macromolecules. 182:838-848.
Feng T, Leptihn S, Dong K, Loh B, Zhang Y, Li M, Guo XK, Zelin Cui (2021) JD419, a Staphylococcus aureus phage with a unique morphology and broad host range. Frontiers in Microbiology (Virology) 12: 602902.
Loh B*, Gondil VS*, Manohar P, Khan FM, Yang H, Leptihn S (2020) Encapsulation and Delivery of Therapeutic Phages. Applied and Environmental Microbiology 87:e01979-20. (Featured on cover)
Fang J, Zhang L, He J, Zhao M, Loh B, Leptihn S, Yu Y, Hua X (2020) Plasmid dynamics of mcr-1-positive Salmonella spp. in a general hospital in China. Frontiers in Microbiology (Antimicrobials, Resistance & Chemotherapy) 11: 3320.
Loh B*, Chen J*, Manohar P, Yu Y, Hua X, Leptihn S (2020) A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length and Genomic Positions. Frontiers in Microbiology (Infectious Diseases) 11: 3055.
Feng T, Leptihn S, Dong K, Loh B, Zhang Y, Li M, Guo X, Cui Z (2020) Characterisation of bacteriophage JD419, a Staphylococcal phage with an unusual morphology and broad host range. bioRxiv 2020.11.09.370866
Hua X, Xu Q, He J, Zhang D, Chen Q, Zhang L, Loh B, Leptihn S, Wen Y, Higgins P, Zhou Z, Yu Y (2020) The distribution of mutations and hotspots in transcription regulators of resistance-nodulation-cell division efflux pumps in tigecycline non-susceptible Acinetobacter baumannii in China. International Journal of Medical Microbiology 310(8): 151464.
Loh B*, Chen J*, Manohar P, Yu Y, Hua X, Leptihn S (2020) A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length and Genomic Positions. bioRxiv
Manohar P, Loh B, Leptihn S (2020) Will the overuse of Antibiotics during the Coronavirus pandemic accelerate Antimicrobial resistance? Infectious Microbes & Diseases 2(3): 87-88.
Manohar P, Loh B, Nachimuthu R, Hua X, Welburn SC, Leptihn S (2020) Secondary Bacterial Infections in Patients with Viral Pneumonia. Frontiers in Medicine (Infectious Diseases – Surveillance, Prevention and Treatment) 7: 420.
Manohar P*, Loh B*, Athira S, Nachimuthu R, Hua X, Welburn SC and Leptihn S (2020) Secondary Bacterial Infections During Pulmonary Viral Disease: Phage Therapeutics as Alternatives to Antibiotics? Frontiers in Microbiology (Antimicrobials, Resistance and Chemotherapy) 11: 1434.
Loh B and Leptihn S (2020) A call for a multidisciplinary future of Phage Therapy to combat multi-drug resistant bacterial infections. Infectious Microbes & Diseases 2(1): 1-2.
Loh B, Kuhn A, Leptihn S (2019) The fascinating biology behind phage display: Filamentous Phage Assembly. Molecular Microbiology 111(5): 1132-1138.
Loh B, Haase M, Mueller L, Kuhn A, Leptihn S (2017) The transmembrane morphogenesis protein gp1 of filamentous phages contains Walker A and Walker B motifs essential for phage assembly. Viruses 9(4): 73.
Altrichter S, Haase M, Loh B, Kuhn A, Leptihn S (2017) Mechanism of the spontaneous and directional membrane insertion of a 2-transmembrane ion channel. ACS Chemical Biology 12(2): 380-388.
Utturkar SM, Huber H, Leptihn S, Loh B, Brown SD, Stetter KO, Podar M (2016) Draft genome sequence of Pyrodictium occultum PL19, a marine hyperthermophilic species of Archaea that grows optimally at 105℃. Genome Announcement 4(1).
Jonas S*, Christie M*, Peter D*, Bhandari D, Loh B, Huntzinger E, Weichenrieder O & Izaurralde E (2014) An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay. Nature Structural & Molecular Biology 21(7): 599-608.
Chen Y*, Boland A*, Kuzuoğlu-Öztürk D*, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E (2014) A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing. Molecular Cell 54(5): 737-50.
Chang CT, Bercovich N, Loh B, Jonas S & Izaurralde E (2014) The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1. Nucleic Acids Research 42: 5217-5233.
Loh B, Jonas S & Izaurralde E (2013) The SMG5-SMG7 heterodimer directly recruits the CCR4-NOT deadenylase complex to mRNAs containing nonsense codons via interaction with POP2. Genes & Development 27: 2125-38.
Bawankar P, Loh B, Wohlbold L, Schmidt S & Izaurralde E (2013) NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex that docks onto the NOT1 N-terminal domain. RNA Biology 10: 228-244.
Vozzolo L, Loh B, Gane PJ, Tribak M, Zhou L, Anderson I, Nyakatura E, Jenner RG, Selwood D & Fassati A (2010) Gyrase B inhibitor impairs HIV-1 replication by targeting Hsp90 and the capsid protein. Journal of Biological Chemistry 285: 39314-28.
Loh B, Vozzolo L, Mok J, Lee C, Fitzmaurice R, Caddick S and Fassati A. (2009) Inhibition of HIV-1 replication by isoxazolidine and isoxazole sulfonamides. Chemical Biology & Drug Design 75(5): 461-74.
Loh B,Wang XW, Bui THH, Luan XL, Ho B andDing JL. (2008) SARM: A novel Toll-like receptor adaptor, is functionally conserved from arthropod to human. Molecular Immunology 45(6):1732-42.